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This study investigated the relationship between personality traits, ambiguity tolerance, and health hardiness in infertile women. Findings indicate that higher levels of ambiguity tolerance and certain personality traits, such as extroversion, agreeableness, and conscientiousness, are associated with greater health hardiness in infertile women. Conversely, higher levels of neuroticism are linked to lower levels of health hardiness. Ambiguity tolerance appears to mediate the relationship between personality traits and health hardiness, suggesting that individuals with higher levels of ambiguity tolerance are more likely to benefit from the protective effects of personality traits on health hardiness. These findings highlight the importance of considering personality and psychological factors in the management of infertility-related stress and well-being.