RNA-seq of AMPKa1-deficient bone-marrow-derived macrophages

To identify cross-talk between the cytoplasmic kinase AMPK and the nuclear receptor, glucocorticoid receptor, during inflammatory responses, bone-marrow-derived macrophages lacking the catalytic domain of AMPK, AMPKa1, were exposed to vehicle, dexamethasone, a synthetic glucocorticoid, lipopolysaccharide, crushed muscle extract (damage-associated molecular patterns) or a combination of the inflammatory stimulus and dexamethasone. Overall design: RNA-seq of BMDMs exposed to vehicle (DMSO), dexamethasone (100nM), lipopolysaccharide (100ng/ml), DAMPs (1ug/ml) or a combination of lipopolysaccharide and dex, or DAMPs and dex for 24 hours before RNA isolation.