Quasispecies analysis of NS5A genomic residues of HCVp0 populations passaged in the absence or presence of ribavirina.

aThe populations analyzed correspond to the infections at an initial MOI of 1 to 2 TCID50/cell described in Fig. 3a. Passage 5 in the infections with 100 μM Rib did not yield sufficient HCV RNA for analysis.The genomic residues analyzed are 6269 to 7666 of the NS5A-coding region. The HCV genome residue numbering corresponds to the JFH-1 genome (accession number #AB047639).bThe parenthesis indicates the number of clones analyzed, followed by the number of haplotypes (number of different RNA sequences); some clones did not contain the full length sequence; when the alignment of the sequenced region was correct such clones were entered in the calculation.cMutation frequency and nucleotide diversity are defined in Table 1 legend and Materials and Methods. Mutation types are summarized in Fig. 4b and their position in the HCV genome and deduced amino acid substitutions are given in Table S4.