Illustration of FRAP data treatment for PvdQ-mCHERRY and comparison of the fast diffusing of PvdS-YFP with the slow diffusing TonB-mCHERRY.

A. One-dimensional profiles along the long cell axis after photobleaching (t = 0 s), during recovery (t = 1 s) and after full recovery (t = 2 s) of strain pvdQ-mcherry. Inset: Fluorescence images extracted from the acquired FRAP stream. From left to right: before photobleaching, after photobleaching and after recovery. B. Experimental Fourier amplitudes for mode n = 1 as a function of time of PvdQ-mCHERRY and the fitted exponential decay (solid line) used to determine the diffusion coefficient. C. Experimental Fourier amplitudes for mode n = 1 as a function of time for PvdS-YFP (gray circles) and TonB-mCHERRY (black circles) and the corresponding fitted exponential decay (hashed and solid lines, respectively).