Strains used in this study.

Abbreviation descriptions. ScSUC2: S. cerevisiae Invertase. OCH1: Alpha-1,6-mannosyltransferase. KlMNN2-2: K. lactis UDP-GlcNAc transporter. BMT1-4: Beta-mannose-transferase genes 1 to 4. MNN4L1: MNN4-like gene 1. MmSLC35A3: Mouse homologue of UDP-GlcNAc transporter. PNO1: Phosphomannosylation of N-linked oligosaccharides. MNN4: Mannosyltransferase (charge elimination). ScGAL10: UDP-glucose 4-epimerase. XB33: Truncated HsGalT1 fused to ScKRE2 leader. DmUGT: UDP-Galactose transporter. KD53: Truncated DmMNSII fused to ScMNN2 leader. TC54: Truncated RnGNTII fused to ScMNN2 leader. NA10: Truncated HsGNTI fused to PpSEC12 leader. FB8: Truncated MmMNS1A fused to ScSEC12 leader. TrMDS1: Secreted T. reseei MNS1. STT3D: oligosaccharyltransferase.