HPLC data of NEREA Augmented Observatory

The water used for the determination of the pigment spectrum by HPLC accounts for 2-3 litres for each depth. The seawater is filtered on GF/F filters (diameter 47mm). The filters are stored in liquid nitrogen until pigments HPLC analysis. HPLC pigments separations were made on an Agilent 1100 HPLC (Agilent technologies, United States). The system was equipped with an HP 1050 photodiode array detector and a HP 1046A fluorescence detector for the determination of chlorophyll degradation products. Instrument calibration was carried out with external standard pigments provided by the International Agency for 14C determination-VKI Water Quality Institute.