Expression data from SUM149 inflammatory breast cancer cells cultured in monolayer or Matrigel

Inflammatory breast cancer (IBC) is a rare type of breast cancer but accounts for up to 10% of breast cancer-related deaths. Plasticity between epithelial and mesenchymal feature is reported to be crucial in metastasis of IBC. Using Matigel culture, we induced epithelial to mesenchymal transition (EMT) in epithelial-like SUM149 IBC cells and identified overexpressed genes in this EMT process. SUM149 cells were plated on monolayer or Matrigel culture (triplicates each) on day 0. On day 2, SUM149 cells on monolayer culutre were collected by tripsinizing them and SUM149 cells on Matrigel culture were recovered by using Cell Recovery Solution for RNA extraction and hybridization on Affymetrix microarrays.