Treadmill physical exercise on prearthritic phase of collagen induced arthritis in DBA1 mice_transcriptome responses on tarsal joint

Gene expression profiles in the tarsal joints of DBA/1 mice whit collagen-induced arthritis that performed treadmill exercise in the pre-arthritic phase vs. those who were not exercised were compared. Exercise induced the downregulation of inflammatroy genes and signaling pathways. Pools of total RNA were made per study group (eight mouse per group): CIA exercised mice (test group) and CIA non-exercised mice (reference). The reverse transcription polymerase chain reaction (RT-PCR) was performed and the resulting complementary DNAs were labeled with Cy5 (CIA exercised mice) or Cy3 (CIA non-exercised mice). Hybridation analysis used the mouse 65-mer oligo library from Sigma-Genosys to compare the expression of 23,232 gene-specific oligonucleotide probes for mouse.