Replication Data for: ATP of Replicative Senescence in Mesenchymal Stem Cells: An In Vitro Study on Mitochondrial Dynamics and Metabolic Alterations"

ATP levels were quantified using the CellTiter-Glo Luminescent Cell Viability Assay (G7571, Promega). This assay is based on a thermostable luciferase that, in the presence of Mg2+, catalyzes an oxidative reaction, generating bioluminescence. Specifically, luciferin, along with molecular oxygen and ATP as cofactors, is converted into oxyluciferin, resulting in light emissions. bMSCs seeded on a 96-well white plate (Costar, Sigma-Aldrich) (6 × 103 cells/well) were incubated with CellTiter-Glo Reagent, diluted in culture medium with a 1:1 ratio, for 10 min at room temperature. The luciferase activity was monitored using the Varioskan LUX Multimode Microplate Reader (Thermo Fisher Scientific). Data were normalized to the cell number.