Superior Expansion of Zone 2 Hepatocytes in Pregnancy

We found that the hepatocyte proliferation wave initiated from zone 1/2 to zone 2/3 during early-to-late pregnancy, resulting in the generation of most new hepatocytes in zone 2. By single-cell RNA sequencing, we identified that Ccnd1 was highly enriched in zone 2. Overall design: Mouse primary hepatocytes were isolated from 8-week-old mice by two-step collagenase perfusion, and analyzed using scRNAseq.