Antiviral RNAi response against the insect specific Agua Salud alphavirus

Small RNA sequencing to profile the interactions between exo-siRNA pathway of Aedes aegypti- derived AF5, Af525 (Ago2 knock-out cell line) and AF319 (Dcr2 knock-out cell line) cells against Agua Salud alphavirus (ASALV). Results showed that ASALV-specific siRNA was still produced in the absence of Ago2 but decreased in the Dcr2 knock-out cell line. ASALV was able to trigger vpiRNA production through the ping-pong production pathway in Dcr2 knock-out cells.