Fig 4. dSTORM images of rhabdomeres

Synd and p-Moe localization at the rhabdomere base in the thinner proximal portion of the isolated ommatidia from late pupal flies observed from the cytoplasmic side by dSTORM. Image frames of 30,000 – 53,000 were denoised and deconvolved to increase resolution in noisy images and reconstructed using DAOSTORM. Sample drift was corrected by iterative cross correlation of time projection of 1,000 frames of the original STORM images. To superimpose the 2-color images, a reference image using bleed through of shorter wavelength was obtained before dSTORM imaging and used to calculate the registration parameters using Chromagnon v0.64 software. Fluorescent spots were placed as 30nm FWHM Gaussian peaks with a pixel size of 10 nm.