The anti-leukemic effect of R-2HG depends on its acting as an m6A mRNA modifier-RNA Seq-Resistant, sensitive and healthy control

To identify the potential genes/signaling pathways related to R-2HG sensitivity in leukemia cells. Overall design: Via MTT assays, we determined the various sensitivities to R-2HG treatment in leukemia cells. We selected five resistant leukemia cells (NB4, TF-1, HEL, MA9.3RAS and K562), for sensitive leukemia cells (ML-2, NOMO-1, U937 and MA9.3ITD) and four healthy control samples (two CD34+ hematopoietic stem/progenitor cells (CD34+ HSPC) and two mononuclear cells (MNC)) for RNA-sequencing. NOTE: The original processed data files were replaced with updated processed data files (containing 'cell line name' column headers, instead of 'sample id') on May 2019 to avoid confusion.