Figure S1 - Mirtazapine Inhibits Tumor Growth via Immune Response and Serotonergic System

(A) The growth curves of parental CT-26 and CT-26/luc tumor cells. Td = (t-t0)×ln2/ (lnN – lnN0). The cell doubling times are 14.2 h and 14.4 h for parental CT-26 and CT26/luc cells, respectively. (B) Left: the luciferase expression in CT-26/luc cells imaged with Xenogen IVIS 50 imaging system. Right: the photon counts emitted from CT-26/luc cells is the function of the cell number with R2 = 0.993. (C) Cell viability analysis of CT26/luc cells treated with various concentrations (0, 5, 10, 20, 40, and 80 µM) of mirtazapine for 24, 48, and 72 h, and measured with MTT assay as described in the “Materials and Methods”. (D) Effect of mirtazapine on the cell cycle of CT-26/luc cells after treatment with various concentrations of mirtazapine for 24 h, and analyzed by flow cytometry. No cytotoxic effect was found.
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