Gene expression analysis of oxidative stress-related genes in A375 melanoma cells over-expressing hMp84.

Gene expression was analyzed in triplicate samples by using RT2 Profiler PCR Array—Human Oxidative Stress. For NCF2, GSTZ1 and CCL5, three additional independent experiments in triplicates were performed by RT-PCR. Gene expression values in p84-cells are expressed as fold-change (mean ± S.E.) over EV-cells after 24 h of transfection. *SOD2 has been included both in genes involved in Reactive Oxygen Species (ROS) production/metabolism and in oxidative stress-responsive genes, encoding antioxidants. Gene expression analysis of oxidative stress-related genes in A375 melanoma cells over-expressing hMp84.