PolyA RNA-seq at 0, 2, 4 and 6h post meiosis induction in S. cerevisiae wild type and rie1 deletion mutant

Across species, mRNA production is shutdown or largely reduced during the process of producing gametes. Thus gametogenesis relies heavily on post-transcriptional regulation. Our goal is to assess which RNA-binding proteins (RBP) containing RNA Recognition Motifs (RRM) are essential for proper entry in meiosis in the budding yeast Saccharomyces cerevisiae. We screened a strain deletion library of non-essential genes coding for RRM-containing RBP for defects in processes leading to the first meiotic division. We characterized the role of one such protein, Rie1. With this SRA dataset, we compared the differential gene expression pattern by polyA RNA-seq in wild type and mutant Rie1 at different time points post sporulation induction.