Knockdown of BmorCPR67 gene disrupts prepupal–pupal transition of silkworm Bombyx mori by thinning the endocuticle

The cuticle of insects serves as a crucial organ for preserving body composition, protecting against pathogen invasion, and retaining moisture in their bodies. Cuticular proteins (CPs) are the main constituents of insect cuticles and interact with chitin to form cuticle structural framework and mechanical properties. In this study, we investigated the role of a cuticular protein with R&R consensus (CPR), BmorCPR67, a member of the RR-2 subfamily, during the prepupal-to-pupal transition in Bombyx mori. The BmorCPR67 gene exhibited high expression levels during the prepupal stage, with the highest expression detected in the epidermis of the day-1 pupa in B. mori. And the expression of BmorCPR67 gene was induced by 20-hydroxyecdysone (20E). Chitin-binding assays indicated that the BmorCPR67 protein selectively binds to crystalline chitin and chitosan but not to amorphous chitin. Silencing BmorCPR67 gene disrupted the molting process from prepupa to pupa, resulting in silkworms mortalit..., , , # Knockdown of BmorCPR67 gene disrupts prepupal–pupal transition of silkworm Bombyx mori by thinning the endocuticle

[https://doi.org/10.5061/dryad.bg79cnpmw](https://doi.org/10.5061/dryad.bg79cnpmw)

## Description of the data and file structure

Knockdown of BmorCPR67 gene disrupts prepupal–pupal transition of silkworm Bombyx mori by thinning the endocuticle

This dataset contains data and analysis code related to the research on the role of BmorCPR67 gene disrupts prepupal–pupal transition of silkworm Bombyx mori. The BmorCPR67 gene exhibited high expression levels during the prepupal stage, with the highest expression detected in the epidermis of the day-1 pupa in B. mori. And the expression of BmorCPR67 gene was induced by 20-hydroxyecdysone (20E). Chitin-binding assays indicated that the BmorCPR67 protein selectively binds to crystalline chitin and chitosan but not to amorphous chitin. Silencing BmorCPR67 gene disrupted the molting process from prepupa to pupa, resulting in silkw...,