Sporozoite numbers and localization in mosquitoes infected with control and mutant parasites.

*Mosquitoes were infected with the indicated parasite clones and midguts, hemolymph and salivary glands were harvested from 15 mosquitoes at days 14, 16 and 18 post-infective blood meal respectively, pooled and sporozoites were counted. Shown are the means of three independent experiments ± SD.#Sporozoites in the supernatant and pellet of trypsin-treated salivary glands were counted and the percentage inside was calculated. There were 15 mosquitoes per group. This experiment was performed twice with similar results.