Medaka_Reciprocal_cross_RNAseq. Medaka_Reciprocal_cross_RNAseq

Medaka fish is a long standing genetic model organism from the 1930s. Uniquely amongst vertebrates Medaka fish can be routinely inbred from the wild (laboratory mice are inbred, but this does not happen as a routine process from wild individuals), leading to a large number of fully inbred wild-derived strains. As part of a broader collaboration I am part of a project to inbred over 100 wild derived strains of Medaka and use them in a similar manner to Arabidopsis and Drosophila wild derived lines. To help explore the phenotyping possibilities in this context, we have taken already established wild Medaka lines and done reciprocal F1 crosses between 3 strains, and have 4 tissues, and a number of parental tissues, giving a total of 40 samples. By doing RNA-seq on these tissues we do a number of things: (a) assess the level of allele specific expression in Medaka (b) test whether there is any imprinting (parent of origin) in fish. This is thought to not be the case, but in fact has not been well tested (not least because getting truly inbred fish is hard) (c) assess the level of random allelic activation in brain (d) assess the feasibility for RNAseq based phenotyping in Medaka, providing preliminary data for future proposals. This data is part of a pre-publication release. For information on the proper use of pre-publication data shared by the Wellcome Trust Sanger Institute (including details of any publication moratoria), please see http://www.sanger.ac.uk/datasharing/