The transgenerational physiological and molecular data of Oryzias melastigma under seawater acidification stress

As shown in previous studies, anthropogenic CO2 emissions are increasing and cause lots of environmental changes, such as global warming; however, this change is also threatening the ocean. Several pieces of evidence pointed out that ocean acidification would impact marine life, especially in the early developmental stages. To understand the long-term and transgenerational effects of marine fish, we applied a marine model animal, Oryzias melastigma, to a CO2-induced acidified condition (pH 7.6). This study focused on the larvae's physiological acclimation and how they face acidified stress through gene expression changes. Furthermore, we checked the epigenetic modifications, like DNA methylation, to know if there were any parental effects under this stress.,

Breeding conditions:
We used the pH controller (E-M03, Macro, Taiwan) to monitor the pH value in each tank and pumped the extra CO2 into the seawater to maintain a low pH condition (pH 7.6). The fertilized eggs from the control tank (WT, pH 8.1) were transferred immediately to acidified seawater until mature (F0), then swapped for the next generation (F1) and kept for the second generation (F2).
The seawater system was kept in a similar condition (salinity 31–33, temperature 28±1 °C, 10 h dark/14 h light cycle) at Marine Research Station (the Institute of Cellular and Organismic Biology, Academia Sinica, Taiwan). During the exposure period, the water conditions were also recorded, like pH, temperature(T, °C), salinity (S, ‰), total alkalinity (TA,μM) and the total dissolved inorganic carbon (DIC,μM) and pCO2 was calculated by CO2SYS module.
The pH and temperature (°C) were measured using a pH meter (pH 3310, WTW, Germany) equipped with a pH electrode (SenTix® 41, WTW, Germany).
TA (μM..., , # The transgenerational physiological and molecular data of Oryzias melastigma under seawater acidification stress

[https://doi.org/10.5061/dryad.4xgxd25j7](https://doi.org/10.5061/dryad.4xgxd25j7)

This data set provides the physiological and molecular results from the control group (WT) and three acidified acclimated generations (F0, F1, and F2). Due to our observations, the marine medaka was impacted by the acidified stress; however, they seemed to overcome this stress and acclimated to the low pH condition over generations.

## Description of the data and file structure

**All raw data is separated, and the information for each file is as follows:**

1. **Om_Growth_parameters.csv**: Records growth parameters (in μm), including interocular distance, eye diameter, total body length, body height, and body width, measured from 2 dpf to 7 dpf.
2. **Om_metabolism.csv**: provide the oxygen consumption (μmol O2/h.g) and ammonium excretion (μmol NH4+ /h.g) at 5 dpf larvae.
3. **Om_Heartbeat...,