viral metagenome Genome sequencing and assembly. viral metagenome

RNA viruses can cause human and animal infectious diseases, leading to animal husbandry and public health problems . Identification and analysis of RNA viruses is important in the diagnosis, treatment, control and prevention of human and animal infectious diseases. Multiple methods have been published for strand-specific RNA-seq on NGS platforms , but it is not certain which library preparation method is better to identify and characterize RNA viruses. Whether known or unknown viruses in biology samples can be discovered and characterized is related to the method of preparing the NGS library. In this study, three methods for preparing NGS libraries were compared to evaluate which method was most suitable for virus identification and characterization.