Adaptive response of Pseudomonas aeruginosa under serial ciprofloxacin exposure

Supplementary Material for 'Adaptive response of Pseudomonas aeruginosa under serial ciprofloxacin exposure', as described in Microbiology. Understanding the evolution of antibiotic resistance is important for combating drug-resistant bacteria. In this work, we investigated the adaptive response of Pseudomonas aeruginosato ciprofloxacin. Ciprofloxacin-susceptible P. aeruginosa ATCC 9027, CIP-E1 which was exposed to ciprofloxacin for 14 days and CIP-E2 strain which was CIP-E1 cultured in antibiotic-free broth for 10 days were comparatively analyzed. Phenotypic response including cell morphology and production of pyoverdine, pyocyanin, and rhamnolipid were assessed. Proteomic responses were evaluated using comparative iTRAQ labeling LC-MS/MS to figure out differentially expressed proteins (DEPs).