RNA sequencing to identify fusion transcripts in late-stage relapse patients with estrogen receptor positive breast cancer

RNA sequencing (RNA-seq) detects estrogen receptor alpha gene (ESR1) fusion transcripts in estrogen receptor positive (ER+) breast cancer but their role in disease pathogenesis remains unclear. We performed sequencing on RNA extracted from metastatic biopsy samples from patients with ER+ breast cancer to assess the presence of fusion transcripts. Overall design: RNA-seq of frozen metastatic biopsy samples from ER+ breast cancer patients.