CONNETTING ANTI-2GLYCOPROTEIN I ANTIBODIES, NETosis AND ENDOTHELIAL CELL ACTIVATION

This project aims to explore the intricate relationship between anti-β2glycoprotein I (aβ2GPI) antibodies, neutrophil extracellular traps (NETs), and endothelial cell activation in Antiphospholipid Syndrome (APS), an autoimmune disorder characterized by thrombotic events. This study tested the hypothesis that aβ2GPI antibodies may both induce NETosis and act as NET-binding antibodies. It further evaluated the ability of aβ2GPI-induced NET to cause endothelial activation, TF expression increase and endothelial disfunction. Furthermore, a proteomics analysis was conducted to compare aβ2GPI-induced NETs with NETs induced by phorbol myristate acetate (PMA). Please note that aβ2GPI-induced NETs are labeled as 'TV' in the raw files, while PMA-induced NETs are labeled as 'T'.