LMO2/LDB1 protein complex in AML cells

These are raw mass spectrometry data discussed in our paper: “The LMO2-LDB1-TAL1 complex regulates transcription networks in AML”, where we implicate this multisubunit complex as a key regulator of leukemia cell transcription. The key method was affinity purification followed by tandem mass spectrometry. The LDB1 and LMO2 proteins were FLAG tagged and encoding cDNAs were cloned into lentiviral expression vectors allowing transduction of leukemia cells. Protein purification and MS/MS are described below.