Hepatic transcriptome profiling of liver-specific Elovl6 knockout mice fed a high-sucrose diet

Elovl6 is a member of mammalian fatty acid elongase that is responsible for converting C16 saturated and monounsaturated fatty acids into C18 species. To understand the role of Elovl6 in liver, we generated liver-specific Elovl6 knockout mice (Albumin-Cre;Elovl6 fl/fl C57BL/6J). The goal of this experiment was to determine effects of hepatocyte Elovl6 deficiency on the murine liver transcriptome under lipogenic condition. Liver-specific Elovl6 knockout (LKO) and control mice (n=3 per group) were fed high-sucrose diet (D11725, Research Diets) for 14 days, fasted for 4 hr, then sacrificed. Livers were removed and flash frozen in liquid nitrogen. RNA was extracted and hybridized to Agilent SurePrint G3 Mouse Gene Expression 8x60K Microarrays.