Complementation tests involving three alleles of bbs-7 reveal complexity in interactions between alleles.

Animals homozygous for the my13 allele do not take up fluorescent dye in their phasmids and exhibit abnormal dye-filling in the amphids (tailDyf) while ok1351 homozygotes and n1606 homozygotes do not take up dye in either the amphids or phasmids (Dyf). Trans-heterozygotes involving the my13 allele (my13/ok1351 and my13/n1606) have a similar phenotype to the my13 homozygotes. ok1351/n1606 trans-heterozygotes are pre-dominantly dye-filling defective. Wild-type animals (him-5(e1490)) were 100% nonDyf (n = 46). The percentage of animals with PKD-2::GFP mislocalization (the Cil phenotype) is less in the trans-heterozygotes compared to animals homozygous for my13 allele.Complementation tests involving three alleles of bbs-7 reveal complexity in interactions between alleles.