Phenotypic characteristics of the <i>fliZ</i> mutant with and without complementation with a <i>fliZ</i>-expressing plasmid^a.

a. All plates were incubated for 2 days at 28°C before assay interpretation, unless otherwise indicated. aTc, anhydrotetracycline; ND, not determined. b. Btb, bromothymol blue; B, dark blue colonies. c. +, zone of Micrococcus luteus (laboratory collection) growth inhibition; median diameter = 35±6 mm. d. +, halo up to 8 mm in diameter. e. T, total hemolysis; P, partial hemolysis, −, no hemolysis on 24 h-old cultures. f. Chemotaxis halo: ++, large spreading area (>20 mm); +, ∼10 mm; −, no spreading area. g. We observed 72 h-old cultures on Tween plates. Qualitative evaluation of the halo surrounding the bacterial colony +, activity detected; −, no halo.