Sensitivities of Antibody and Viral RNA Detection in Fecal Samples From Captive and Wild Chimpanzees.

aWestern blot strips were prepared using an infectious molecular clone (pMod-1) of SFVcpzPts (see Methods).bRT-PCR was performed using SFVcpz specific pol-IN primers.cSensitivities of SFVcpz antibody and viral RNA detection were determined for captive (YK) as well as wild-living chimpanzees at different field sites (TA, DP, EK, BB, MB, LB, GT, GM, MH).dSFVcpz infection in captive chimpanzees was confirmed by demonstrating virus specific antibodies in their blood; SFVcpz infection of wild-living chimpanzees was determined by demonstrating virus specific antibodies or viral RNA in at least one fecal sample.eThe sensitivities of fecal antibody and viral RNA detection were calculated for each site based on the total number of samples collected from infected chimpanzees at that site (with 95% confidence intervals [CI]); the specificity of fecal antibody detection was determined by testing fecal samples from uninfected human volunteers (Table 1) and determined to be 1.00 (0.87�C1.00); the specificity of virion RNA detection was set to 1.00 since all amplification products were sequence confirmed.