Single-Cell RNA-Seq analysis of diabetic wound CD45+ cells in STZ-induced mice

The crucial role of immune cells in the healing of chronic diabetic wounds is widely known, but previous in vitro classification and marker genes of immune cells may not be fully applicable to cells in the microenvironment of chronic wounds. The heterogeneity of immune cells was studied and classified at the single-cell level in a chronic wound model. We performed single-cell sequencing of CD45+ immune cells within the wound edge and obtained 17 clusters of cells, including 4 clusters of macrophages. This project has provided an important reference for the study of immune-related mechanisms in diabetic chronic wounds. STZ-induced C57Bl6j mice diabetic wound and control wound CD45+ cells.Single cell RNA sequencing was performed with 10XGenomics.