The genomic and transcriptional landscape of primary central nervous system lymphoma

Primary lymphomas of the central nervous system (PCNSL) are mainly diffuse large B-cell lymphomas (DLBCLs) confined to the central nervous system (CNS). Despite extensive research, the molecular alterations leading to PCNSL have not been fully elucidated. In order to provide a comprehensive description of the genomic and transcriptional landscape of PCNSL, we here performed whole-genome and transcriptome sequencing and integrative analysis of 51 lymphomas presenting in the CNS, including 42 EBV-negative PCNSL, 6 secondary CNS lymphomas (SCNSL) and 3 EBV+ CNSL and matched controls. The results were compared to an independent validation cohort of 31 FFPE CNSL specimens (PCNSL, n = 19; SCNSL, n = 9; EBV+ CNSL, n = 3) and 36 systemic DLBCL cases outside the CNS. This repository contains tab separated value text files: - Radke_et_al_supplementary_somatic_CNVs.tsv (somatic copy number variations predicted by ACEseq)<br> - Radke_et_al_supplementary_somatic_indels.tsv (somatic indels predicted by the DKFZ platypus workflow)<br> - Radke_et_al_supplementary_somatic_indels_exonic.tsv (somatic exonic indels predicted by the DKFZ platypus workflow)<br> - Radke_et_al_supplementary_somatic_mutations_integrated.tsv (table of gene by patients, stating which mutations were observed) <br> - Radke_et_al_supplementary_somatic_mutations_integr_integrated_including_kataegis_counts.tsv (table of gene by patients, stating which mutations were observed, including the count of mutations falling into kataegis hotspots) <br> - Radke_et_al_supplementary_somatic_SNVs.tsv (somatic SNVs predicted by the DKFZ mpileup workflow)<br> - Radke_et_al_supplementary_somatic_SNVs_exonic_functional.tsv (somatic exonic SNVs predicted by the DKFZ mpileup workflow)<br> - Radke_et_al_supplementary_somatic_SNVs_rescued_by_TiNDA (mutations initially classified as germline, but likely tumor mutations based on VAF modelling by TiNDA)<br> - Radke_et_al_supplementary_somatic_SVs.tsv (somatic structural variations predicted by the DKFZ Sophia workflow)<br> - Radke_et_al_supplementary_RNAseq_numReads_CNSLs.tsv (RNAseq read counts calculated by the DKFZ RNAseq workflow) This repository contains the raw unedited images from the manuscript: - Radke_et_al_Main_Figure_1c_BCL6.tif (raw unedited image for Main Figure 1c - BCL6)<br> - Radke_et_al_Main_Figure_1c_CD10.tif (raw unedited image for Main Figure 1c - CD10)<br> - Radke_et_al_Main_Figure_1c_MUM1.tif (raw unedited image for Main Figure 1c - MUM1)<br> - Radke_et_al_Supplementary_Figure_1a_CD20.tif (raw unedited image for Supplementary Figure 1a - CD20)<br> - Radke_et_al_Supplementary_Figure_1a_EBV.tif (raw unedited image for Supplementary Figure 1a - EBV)<br> - Radke_et_al_Supplementary_Figure_1a_HE_(FFPE).tif (raw unedited image for Supplementary Figure 1a - HE (FFPE))<br> - Radke_et_al_Supplementary_Figure_1a_HE_(frozen)_1.tif (raw unedited image for Supplementary Figure 1a - HE (frozen) 1)<br> - Radke_et_al_Supplementary_Figure_1a_HE_(frozen)_2.tif (raw unedited image for Supplementary Figure 1a - HE (frozen) 2)<br> - Radke_et_al_Supplementary_Figure_1a_HE_(frozen)_3.tif (raw unedited image for Supplementary Figure 1a - HE (frozen) 3)<br> - Radke_et_al_Supplementary_Figure_1a_Ki67.tif (raw unedited image for Supplementary Figure 1a - Ki67)<br> - Radke_et_al_Supplementary_Figure_1b_EBV_PCR.pptx (raw unedited image for Supplementary Figure 1b - EBV PCR )<br> - Radke_et_al_Supplementary_Figure_1c_CDKN2A_FISH_1.jpg (raw unedited image for Supplementary Figure 1c - CDKN2A FISH 1)<br> - Radke_et_al_Supplementary_Figure_1c_CDKN2A_FISH_2.jpg (raw unedited image for Supplementary Figure 1c - CDKN2A FISH 2)<br> - Radke_et_al_Supplementary_Figure_7h_PD-L1_LS-033.tif (raw unedited image for Supplementary Figure 7h - PD-L1 LS-033)<br> - Radke_et_al_Supplementary_Figure_7h_PD-L1_LS-031.tif (raw unedited image for Supplemnetary Figure 7h - PD-L1 LS-031)