Single molecule image sequences of Thermothielavioides terrestris AA9E (TtAA9E) on cellulose in oxygen scavenging systems

Purified and Cy5-labeled lytic polysaccharide monooxygenase (LPMO) Thermothielavioides terrestris AA9E (TtAA9E) interacting at the surface of crystalline cellulose fibrils were imaged by Total Internal Reflectance Fluorescence Microscopy (TIRFM) in two different oxygen scavenging systems: Glucose Oxidase-Catalase (GODCAT) and Protocatechuic Acid/Protocatechuate-3,4-dioxygenase (PCA/PCD). The datasets (movies) show how oxygen and hydrogen peroxide availability, and reductant type/availability impact specific binding of the LPMO to highly ordered cellulose fibrils.