Average results of the evaluation of decellularization efficiency, semi-quantitative analysis of tissue structure and quantitative analysis of fibrillar and non-fibrillar ECM components in control non-decellularized intestine and in all decellularization global groups.

Nuclear cell removal and the staining signal for picrosirius, Gomori reticulin, PAS and Alcian blue correspond to percentages in reference of control non-decellularized intestine samples and are expressed as means plus/minus standard deviations. Tissue DNA quantification is shown as nanograms of DNA per milligram of dry weight of tissue. HE results are shown as median values and quartiles Q1–Q3 and correspond to the following classification: (0) highly organized tissue, (1) low levels of disorganization, (2) intermediate levels of disorganization, (3) high tissue disorganization. The analysis of tissue structure by SEM (interfibrillar spaces and collagen fibers) is shown in a scale ranging between (−) (small and regular interfibrillar spaces and highly disorganized fibers and disrupted three-dimensional structure) and (+++) (very large and irregular interfibrillar spaces and highly organized fibers and adequate three-dimensional structure) as defined in the methods section.