Figure S1 - Expression of Intracellular Interferon-Alpha Confers Antiviral Properties in Transfected Bovine Fetal Fibroblasts and Does Not Affect the Full Development of SCNT Embryos

Schematic of the plasmid pIRESneo-IFN-bCP-LFCIN B-EGFP. The backbone of the vector is pIRSEneo. IFN-NEO cassette: CMV is the promoter of IFN and NEO; LFCIN B gene cassette: goat β-casein regulatory sequence is the promoter of LFCIN B; EGFP gene cassette: CMV is the promoter of EGFP. All restriction sites are shown. Amp, ampicillin resistance gene; IVS, synthetic intron; IRES, internal ribosome entry site of encephalomyocarditis virus; Neo, neomycin phosphotransferase gene; polyA, a fragment of bovine growth hormone poly(A) signal; EGFP, enhanced green fluorescent protein; IFN, β-interferon gene. (DOCX)