Single-cell RNA-Seq Analysis of Retinal Development Identifies NFI Factors as Regulating Mitotic Exit and Late-Born Cell Specification

Single cell RNA sequencing using either an adapted Smart-seq2 protocol on Chx10-GFP (+) retinal progenitor cells; 10x Genomics Chromium Single Cell system across 10 timepoints of mouse retinal development to examine retinal progenitor cell heterogeneity across retinal development and global changes in gene expression from early retinal neuroepithelial cells through specification and differentiation of retinal cell types; 10X Genomics Chromium Single Cell on P14 Nfia/b/x het control or Nfia/b/x tCKO (Chx10-Cre-GFP) retinas Examination of transcript expression within 120,840 cells across 10 developmental time-points (14 experiments) via 10x Genomics and 864 cells via an adapted Smart-Seq2 protocol; Characterization of Nfia/b/x mutant phenotypes using single-cell RNA-seq本数据集包含两类单细胞RNA测序（single cell RNA sequencing）实验，具体如下：其一为采用改良Smart-seq2（Smart-seq2）方案，针对Chx10-GFP阳性（Chx10-GFP (+)）视网膜祖细胞（retinal progenitor cells）开展的测序，共获取864个细胞的转录组数据；其二为借助10x Genomics Chromium单细胞系统（10x Genomics Chromium Single Cell system），覆盖小鼠视网膜发育的10个时间点、完成14组实验的测序，共检测120,840个细胞的转录表达情况，旨在解析视网膜发育过程中视网膜祖细胞的异质性，以及从早期视网膜神经上皮细胞（early retinal neuroepithelial cells）经视网膜细胞类型特化与分化全过程的基因表达全局变化。此外，数据集还包含针对出生后第14天（P14）的Nfia/b/x杂合对照或Nfia/b/x条件性敲除（tCKO, Chx10-Cre-GFP）视网膜的10x Genomics Chromium单细胞测序数据，用于通过单细胞RNA-seq表征Nfia/b/x突变体的表型。