Expression data from peripheral human T cells stimulated with an anti-CD96 mAb

The role of CD96 in human T cells is ambiguous. We demonstrated that engagement of CD96 on human T cells by antibodies provides a strong activating signal dependent on antibody isotype and cross-linking by a subset of Fc? receptors. Here we used RNASeq profiling to analyze the gene expression programme associated with anti-CD96 mAb treatment in purified T cells isolated from the blood of healthy donors. We showed that multiple hallmarks associated with an activated T-cell signature were significantly enriched in the anti-CD96 treatment group. These included gene signatures associated with cell cycle progression, metabolic reprogramming, effector differentiation, and sustained proliferation and survival. Using pathway analysis, we identified several candidate molecules that could mediate signaling downstream of CD96. Overall design: RNASeq profiling of purified T cells isolated from peripheral blood mononuclear cells of 3 healthy donors, and stimulated for 6 hours with plate-bound anti-CD3 mAb and either plate-bound anti-CD96 mAb or a matching isotype control antibody.