Effects of mulching and ridge tillage on microbial diversity in potato fields

This study selected potato fields under natural rainfall conditions in Wuchuan County, China, as experimental sites. Four treatments were established: flat tillage with mulching, ridge tillage with mulching, flat tillage without mulching, and ridge tillage without mulching.Real-time quantitative PCR (q-PCR) was employed to quantify the abundances of nitrifying and denitrifying bacteria related to N2O emissions at various stages. Illumina high-throughput sequencing was used to investigate microbial community structure by targeting 16S rRNA genes, and relevant soil elements were measured.