Chromosome-level genome assemblies of the migratory locust and the desert locust-6

A combination of three sequencing technologies including long-read sequencing (PacBio Sequel), optical mapping (Bionano Saphyr), and chromosome conformation capture technique based on proximity ligation (Hi-C) was applied to construct the chromosome-level genome assembly of the migratory locust (Locusta migratoria). Moreover, short-read DNA-seq (Illumina Data) both from female and male locusts was used to correct assembly errors. To predict protein-coding genes, we further generated short-read RNA-seq and PacBio ISO-seq cDNA data obtained from 11 different tissues and organs of the migrator locust. To track the chromosome dynamics of X chromosome within Orthoptera, we also generated a chromosome-level genome of the desert locust (Schistocerca gregaria) using Nanopore long-read sequencing and Illumina data with Hi-C scaffolding.

本研究采用长读长测序（long-read sequencing，PacBio Sequel）、光学图谱（Bionano Saphyr）以及基于邻近连接的染色体构象捕获技术（Hi-C）三种测序技术，构建了飞蝗（Locusta migratoria）的染色体水平基因组组装。此外，研究还利用取自雌性和雄性飞蝗的短读长DNA测序数据（Illumina Data）对基因组组装结果进行了错误校正。为预测蛋白质编码基因，本研究进一步生成了取自飞蝗11种不同组织器官的短读长RNA测序数据及PacBio ISO-seq cDNA数据。为探究直翅目（Orthoptera）内X染色体的动态变化规律，本研究还利用纳米孔长读长测序技术、Illumina数据结合Hi-C基因组挂载技术，构建了沙漠蝗（Schistocerca gregaria）的染色体水平基因组。

搜集汇总

背景与挑战

背景概述

该数据集提供了飞蝗和沙漠蝗的染色体级别基因组组装，采用PacBio长读长测序、光学图谱和Hi-C技术等多种先进方法构建，并包含11种组织的转录组数据用于基因预测，特别关注直翅目X染色体的动态研究。