Fluctuating reproductive isolation and stable ancestry structure in a fine-scaled mosaic of hybridizing Mimulus monkeyflowers

Hybridization among taxa impacts a variety of evolutionary processes from adaptation to extinction. We seek to understand both patterns of hybridization across taxa and the evolutionary and ecological forces driving those patterns. To this end, we use whole-genome low-coverage sequencing of 458 wild-grown and 1565 offspring individuals to characterize the structure, stability, and mating dynamics of admixed populations of Mimulus guttatus and Mimulus nasutus across a decade of sampling. In three streams, admixed genomes are common and a M. nasutus organellar haplotype is fixed in M. guttatus, but new hybridization events are rare. Admixture is strongly unidirectional, but each stream has a unique distribution of ancestry proportions. In one stream, three distinct cohorts of admixed ancestry are spatially structured at ~20-50m resolution and stable across years. Mating system provides almost complete isolation of M. nasutus from both M. guttatus and admixed cohorts, and is a partial barr..., For full collection and processing details, see the associated manuscript and the analysis code available at https://github.com/mfarnitano/CAC_popgen.
Along three streams in the Columbia River Gorge area, Washington, USA, 0.5x0.5m square plots were laid down where Mimulus species were growing, during flowering seasons (April through June) of 2012, 2019, 2021, and 2022 (note that not all streams were sampled every year, for details see the associated manuscript).
At each plot for the years 2012, 2019, and 2022, phenological data was recorded: the total number of open flowers within each plot was counted every few days for the duration of the flowering season. 
In addition, leaf or bud tissue was collected for sequencing from wild samples of Mimulus guttatus, Mimulus nasutus, and admixed individuals, as well as the allopolyploid species Mimulus sookensis, growing in these streams during the years 2019, 2021, and 2022. For a subset of these wild samples in 2019 and 2022..., , # Data from: Fluctuating reproductive isolation and stable ancestry structure in a fine-scaled mosaic of hybridizing Mimulus monkeyflowers

This dataset provides sample-level and plot-level metadata and processed data related to the analysis of admixture structure and reproductive isolation across space and time within a hybridizing population of Mimulus guttatus and Mimulus nasutus monkeyflowers.

Plot-level observations and sample collections took place during 2019, 2021, and 2022 flowering seasons (April to June) at two sites near the Columbia River Gorge, Washington, USA, named Catherine Creek (CAC) and Little Maui (LM). Tissue was collected from wild samples for sequencing. For some wild samples, offspring seeds were also collected and germinated in the UGA Botany greenhouses for tissue collection and sequencing. Field data and collections were done by Andrea Sweigart and Keith Karoly. Offspring processing, tissue preparation, sequencing, and analysis was done primarily by Matthew ...,