CA activity of CAH1 protein is affected by N-glycosylation.

Rate constants (k, min−1) for monoexponential decay of the normalized 12C18O18O concentration (Figure 7) after injection of air-saturated H218O into H216O buffer without (Buffer) or with HA immunoprecipitate from non-transformed culture cells (WT), or cells stably expressing HC protein in the absence (HC) or presence of tunicamycin (HC+Tun). Reactions were performed at 20°C, pH 6.5 (mean ± SE, n = 3).