Effects of Overexpression of Amino Acid Homorepeat (PolyX) Fusion Proteins on Budding Yeast Cells

To investigate the effects of overexpression of amino acid homorepeat (PolyX) fusion proteins on budding yeast cells, we analyzed the transcriptional responses elicited by overexpression of PolyX-fused fluorescent proteins (EGFP-PolyX). Overexpression of multiple PolyX variants induces toxicity in yeast cells, whereas certain PolyX fusion proteins exert beneficial effects. For example, overexpression of a polyisoleucine-fused protein (PolyI-EGFP) causes growth inhibition, indicating high toxicity, whereas overexpression of a polyaspartate-fused protein (PolyD-EGFP) results in growth rate comparable to that of EGFP alone, indicating low toxicity. In contrast, overexpression of a polyglutamate-fused protein (PolyE-EGFP) enhances growth rate relative to EGFP alone, thereby conferring a beneficial effect. This dataset presents RNA-seq results aimed at elucidating the transcriptional responses induced by overexpression of PolyI-, PolyE-, and PolyD-fused fluorescent proteins.