Single-cell type transcriptome analysis of maize roothair

We investigated root hair-specific transcriptome using RNA-seq in maize. ZmLRL5 was further identified as a key regulator of maize root hair elongation. The maize inbred line B73 were germinated in moist germination paper. 3-day old uniform seedlings were dipped into liquid N and root hairs were scraped off and collected. The remaining root hair less roots were collected. From Total RNA was extracted using the Qiagen RNeasy Plant kit. Libraries were constructed and sequenced on an Illumina HiSeq2500