We find that pre-crRNAs comprising a full-length direct repeat (full-DR-crRNA) sequence with specific stem-loop G-C base substitutions exhibit increased editing efficiencies compared with the standard
1Fraction transformations that gave rise to HygR colonies. 2Fraction transformations that showed loss of DNA methylation in Southern blotting. 3One transformant out of 12 tested exhibited hypomethylat
CRISPR gene editing and control systems continue to emerge and inspire novel research and clinical applications. Advances in CRISPR performance such as optimizing the duration of activity in cells, ti
