SOP 01 Analysis of hydroxy- and nitro-hydroxy-polycyclic aromatic hydrocarbons in animal tissues by HPLC-MS/MS

Standard Operating Procedure (SOP) for extraction of OH-PAHs and Nitro-OH-PAHs from animal tissue using organic solvents, followed by analysis of the extracts by HPLC MS/MS. This document was prepared by the team of the research project "Integrated systemic detection of pollutants in the human body" (INSYDE-HU), n. P2022RJCHC, CUP: H53D23010450001, funded by funded by the PRIN 2022 framework of the Italian National Recovery and Resilience Plan (PNRR).