In vivo experimental evolution of ICC180, a bioluminescent derivative of the mouse enteropathogen Citrobacter rodentium

In these experiments, we set up 10 transmission chains in which C. rodentium transmitted naturally from mouse to mouse. Experiments were performed as described in the collection provided on Protocols.io (1). In addition, nalidixic acid was added as a treatment for five of the transmission chains. To do this, mice were given nalidixic acid in their drinking water at a final concentration of 10 ug/mL. This water was changed every 2-3 days, adding fresh nalidixic acid each time. 

This dataset contains the weights (g), bacterial viability (as colony forming units [CFU] per gram of stool and as relative light units per gram of stool), and bioluminescence from abdomen and rectum (as photons per second).

1. Read, Patel, & Wiles (2022). Initiating and monitoring natural infection of mice by bioluminescent Citrobacter rodentium. protocols.io. https://www.protocols.io/view/initiating-and-monitoring-natural-infection-of-mic-81wgb6qnnlpk/v1

在本项实验中，我们构建了10条自然传播链，其中C. rodentium从鼠到鼠的自然传播过程得以实现。实验操作依照在Protocols.io上提供的汇编描述进行（1）。此外，对于其中五条传播链，我们添加了萘啶酸作为治疗手段。具体操作为，将萘啶酸添加至饮用水中，最终浓度达到10微克/毫升。每隔2-3天更换一次水，每次均添加新鲜萘啶酸。本数据集包含体重（克）、细菌存活率（以每克粪便的菌落形成单位[CFU]和每克粪便的相对光单位表示），以及腹部和直肠的生物发光（以每秒光子数表示）。

1. Read, Patel, & Wiles (2022). 通过生物发光大肠杆菌C. rodentium启动和监测小鼠的自然感染。protocols.io. https://www.protocols.io/view/initiating-and-monitoring-natural-infection-of-mic-81wgb6qnnlpk/v1