Transcriptomic analysis of senescent cells upon PTBP1 knockdown.
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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IMR90 ER:RAS cells were stably transduced with either an empty vector or 2 deconvoluted shRNAs targeting PTBP1. Following selection with puromycin, the cells were treated with 4OHT to induce senescence. 6 days later the cells were collected for total mRNA analysis. PTBP1 is a regulator of alternative splicing. Our previous experiments had shown that PTBP1 depletion inhibits the expression of pro-inflammatory genes without affecting other senescence-associated phenotypes. By performing RNA-seq we confirmed those observations at a global level and analysed how PTBP1 knockdown alters alternative splicing as a potential mechanism of action.
提供机构：
MRC London Institute of Medical Sciences
创建时间：
2022-02-20
