Abnormal skeletal and cardiac development, cardiomyopathy, muscle atrophy and cataracts in mice with a targeted disruption of the () gene-3

Nd 9 days (B, F) in culture showing enhanced cartilage differentiation in -/- cells, with the dashed line in B and F marking the extent of the micromass. Alkaline phosphatase staining after 5 days (C, G) and 9 days (D, H) in culture showing enhanced osteoblast differentiation in -/- cells. I-L: Morphology of monolayer PEF cultures derived from wild type (I, J) and -/- (K,L) embryos with (I, K) and without (J, L) phase contrast. Alkaline phosphatase staining after 10 days in culture (I, J, K, L) showing osteoblast differentiation in the -/- PEFs but not in the wild type PEFs. Scale bar in I-N = 10 μm. M: Semi quantitative RT-PCR of mRNA from wild type (+/+) and -/- PEFs using primers for alkaline phosphatase, collagen I and osteocalcin. Two fold serial dilutions of cDNA were used and normalised to .<b>Copyright information:</b>Taken from "Abnormal skeletal and cardiac development, cardiomyopathy, muscle atrophy and cataracts in mice with a targeted disruption of the () gene"http://www.biomedcentral.com/1471-213X/8/18BMC Developmental Biology 2008;8():18-18.Published online 20 Feb 2008PMCID:PMC2275724.