Assessing the role of RHIM proteins in RIPK3-transcription in MEFs
收藏NIAID Data Ecosystem2026-05-02 收录
下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE281136
下载链接
链接失效反馈官方服务：
资源简介：
We previously have found that RIPK3 induces robust anti-viral and inflammatory transcriptional signaling in death-resistant MEFs, however the molecular mechanism that governs this transcription is not well understood. Here we found that RIPK3-depedent transcription is RIPK1 and TRIF dependent in MEFs. Ripk3-2xFv immortalized mouse embryonic fibroblasts (MEFs) lacking MLKL, were treated with siRNA targeting ZBP1, TRIF, RIPK1 and a scramble encoded control. Activatable Ripk3-MEFs were then treated with BB homodimerizer for 4hrs. MEF RNA lysates were then submitted for bulk RNAsequencing. NOTES: AP1 = BB, qVD= pan caspase inhibitor, RXMM = ripk3-2xFVfl/fl:ubcERT2cre+MLKL-/-
创建时间：
2025-05-07
