Diatom sedimentary ancient DNA metabarcoding from the continental slope of Kamchatka. SO201-2-12KL_diat_sedaDNA

In this study we use sedimentary ancient DNA metabarcoding from the Kastenlot core SO201-2-12KL which was retrieved from the continental slope of Kamchatka near Kronotsky Peninsula (subarctic North Pacific) from which we collected 54 samples. Total DNA was extracted from approximately 4ml sediment per sample. Each extraction batche was composed of 9 samples and one extraction blank. Total DNA was concentrated and diluted to 3 ng/µl. For each batch we performed PCRs in triplicates including a PCR no template control (NTC). We amplified a diatom-specific, 76 bp long part of the rbcL gene with tagged primers Diat_rbcL_705F (AACAGGTGAAGTTAAAGGTTCATAYTT) and Diat_rbcL_808R (TGTAACCCATAACTAAATCGATCAT). The PCR-products were purified and pooled in equal concentrations. The sequencing library was prepared with the Mid Output kit v. 2 according to the Fasteris Metafast protocol for low complexity amplicon sequencing and checked by qPCR. The library was sequenced (2 x 150 bp, paired-end) on the Illumina NextSeq 500 at the Fasteris SA sequencing service (Switzerland). Here, we provide the reads with our tagged primers removed for all sequenced PCR-products including extraction blanks and NTCs.The sample names are composed as follows: Depth_cm_PCRproductID_forwardTag_reverseTag_trimmed. The PCRproductID contains the experiment number (e.g. HZ250P) so that negative controls can be associated with the samples of the corresponding PCR run. We trimmed the forward and reverse tagged-primers, so only the amplicon remains.