Suppressor screen for gad8 ts mutant in fission yeast

The Akt-related Gad8 kinase is required for the fission yeast Schizosaccharomyces pombe cells to proliferate under glucose-limited conditions. While the high-affinity hexose transporter Ght5 translocates to the cell surface in wild-type cells under glucose limitation, gad8-deficient cells exhibit defects in targeting Ght5 to the cell surface. To understand how Gad8 and its upstream, target of rapamycin complex 2 (TORC2), regulate localization of Ght5, we employed next-generation whole genome sequencing and identified extragenic suppressors that restored cell-surface localization of Ght5 in gad8 temperature-sensitive (ts) mutant cells.